Anderson, C;
Khan, MAF;
Wong, F;
Solovieva, T;
Oliveira, NMM;
Baldock, RA;
Tickle, C;
... Stern, CD; + view all
(2016)
A strategy to discover new organizers identifies a putative heart organizer.
Nature Communications
, 7
(12656)
10.1038/ncomms12656.
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Abstract
Organizers are regions of the embryo that can both induce new fates and impart pattern on other regions. So far, surprisingly few organizers have been discovered, considering the number of patterned tissue types generated during development. This may be because their discovery has relied on transplantation and ablation experiments. Here we describe a new approach, using chick embryos, to discover organizers based on a common gene expression signature, and use it to uncover the anterior intestinal portal (AIP) endoderm as a putative heart organizer. We show that the AIP can induce cardiac identity from non-cardiac mesoderm and that it can pattern this by specifying ventricular and suppressing atrial regional identity. We also uncover some of the signals responsible. The method holds promise as a tool to discover other novel organizers acting during development.
| Type: | Article |
|---|---|
| Title: | A strategy to discover new organizers identifies a putative heart organizer |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/ncomms12656 |
| Publisher version: | http://dx.doi.org/10.1038/ncomms12656 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, IN-SITU HYBRIDIZATION, MYOSIN HEAVY-CHAIN, CHICK-EMBRYO, NEURAL INDUCTION, ANTERIOR ENDODERM, SONIC-HEDGEHOG, AVIAN EMBRYOS, HENSENS NODE, CARDIAC MORPHOGENESIS, PRECARDIAC MESODERM |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1514229 |
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