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Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells

Lewis, AT; Gaifulina, R; Isabelle, M; Dorney, J; Woods, ML; Lloyd, GR; Lau, K; ... Thomas, GM; + view all (2017) Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells. Journal of Raman Spectroscopy , 48 (1) pp. 119-125. 10.1002/jrs.4980. Green open access

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Abstract

Raman spectroscopy (RS) is a powerful technique that permits the non‐destructive chemical analysis of cells and tissues without the need for expensive and complex sample preparation. To date, samples have been routinely mounted onto calcium fluoride (CaF_{2}) as this material possesses the desired mechanical and optical properties for analysis, but CaF_{2} is both expensive and brittle and this prevents the technique from being routinely adopted. Furthermore, Raman scattering is a weak phenomenon and CaF2 provides no means of increasing signal. For RS to be widely adopted, particularly in the clinical field, it is crucial that spectroscopists identify an alternative, low‐cost substrate capable of providing high spectral signal to noise ratios with good spatial resolution. Results show that these desired properties are attainable when using mirrored stainless steel as a Raman substrate. When compared with CaF_{2}, data show that stainless steel has a low background signal and provides an average signal increase of 1.43 times during tissue analysis and 1.64 times when analyzing cells. This result is attributed to a double‐pass of the laser beam through the sample where the photons from the source laser and the forward scattered Raman signal are backreflected and retroreflected from the mirrored steel surface and focused towards collection optics. The spatial resolution on stainless steel is at least comparable to that on CaF_{2} and it is not compromised by the reflection of the laser. Steel is a fraction of the cost of CaF_{2} and the reflection and focusing of photons improve signal to noise ratios permitting more rapid mapping. The low cost of steel coupled with its Raman signal increasing properties and robust durability indicates that steel is an ideal substrate for biological and clinical RS as it possesses key advantages over routinely used CaF_{2}

Type: Article
Title: Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/jrs.4980
Publisher version: http://dx.doi.org/10.1002/jrs.4980
Language: English
Additional information: © 2016 The Authors. Journal of Raman Spectroscopy Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Raman spectroscopy, signal improvement, tissue
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Pathology
URI: https://discovery.ucl.ac.uk/id/eprint/1508776
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