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Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models

Liyanage, SE; Gardner, PJ; Ribeiro, J; Cristante, E; Sampson, RD; Luhmann, UFO; Ali, RR; (2016) Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Experimental Eye Research , 151 pp. 160-170. 10.1016/j.exer.2016.08.007. Green open access

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Abstract

Myeloid cells make a pivotal contribution to tissue homeostasis during inflammation. Both tissue-specific resident populations and infiltrating myeloid cells can cause tissue injury through aberrant activation and/or dysregulated activity. Reliable identification and quantification of myeloid cells within diseased tissues is important to understand pathological inflammatory processes. Flow cytometry is a valuable technique for leukocyte analysis, but a standardized flow cytometric method for myeloid cell populations in the eye is lacking. Here, we validate a reproducible flow cytometry gating approach to characterize myeloid cells in several commonly used models of ocular inflammation. We profile and quantify myeloid subsets across these models, and highlight the value of this strategy in identifying phenotypic differences using Ccr2-deficient mice. This method will aid standardization in the field and facilitate future investigations into the roles of myeloid cells during ocular inflammation.

Type: Article
Title: Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.exer.2016.08.007
Publisher version: http://dx.doi.org/10.1016/j.exer.2016.08.007
Language: English
Additional information: Copyright © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Keywords: Science & Technology, Life Sciences & Biomedicine, Ophthalmology, Myeloid cells, Retinal microglia, Neutrophils, Monocytes, Flow cytometry, Experimental autoimmune uveoretinitis, Endotoxin-induced uveitis, Choroidal neovascularization, Dendritic Cells, Choroidal Neovascularization, Macrophage Heterogeneity, Monocytes, Microglia, Activation, Expression, Diversity, Subsets, Reveals
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/1508660
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