Loraine, J;
Pu, F;
Turapov, O;
Mukamolova, GV;
(2016)
Development of an in vitro assay for detection of drug induced Resuscitation-promoting factor dependent mycobacteria.
Antimicrobial Agents and Chemotherapy
, 60
(10)
pp. 6227-6233.
10.1128/AAC.00518-16.
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Abstract
Tuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation of the numbers of viable mycobacteria in treated samples. We generated M. smegmatis strains overexpressing M. tuberculosis resuscitation-promoting factors (Rpfs) and demonstrated their application for the detection of Rpf-dependent mycobacteria generated after drug exposure. Our data offer novel opportunities for validation of the sterilizing activity of antituberculosis agents.
| Type: | Article |
|---|---|
| Title: | Development of an in vitro assay for detection of drug induced Resuscitation-promoting factor dependent mycobacteria |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1128/AAC.00518-16 |
| Publisher version: | http://doi.org/10.1128/AAC.00518-16 |
| Language: | English |
| Additional information: | Copyright © 2016 Loraine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1508457 |
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