Aleksandrova, A;
Czirók, A;
Szabó, A;
Filla, MB;
Hossain, MJ;
Whelan, PF;
Lansford, R;
(2012)
Convective tissue movements play a major role in avian endocardial morphogenesis.
Developmental Biology
, 363
(2)
pp. 348-361.
10.1016/j.ydbio.2011.12.036.
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Abstract
Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.
| Type: | Article |
|---|---|
| Title: | Convective tissue movements play a major role in avian endocardial morphogenesis |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ydbio.2011.12.036 |
| Publisher version: | http://dx.doi.org/10.1016/j.ydbio.2011.12.036 |
| Language: | English |
| Additional information: | Copyright © 2012. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher. |
| Keywords: | Endocardium; Embryo; Morphogenesis; Quail; Time-lapse; Cardiovascular; Imaging |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1508163 |
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