Carlos, MI;
Schätzlein, A;
Uchegbu, I;
(2016)
Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.
In: Candiani, G, (ed.)
Non-Viral Gene Delivery Vectors: Methods and Protocols: Part I.
(pp. 149-157).
Springer New York: New York, NY, USA.
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Abstract
Gene silencing may be achieved by harnessing the RNA interference mechanism to effect down-regulation of protein expression. The therapeutic use of siRNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA by N-(2-ethylamino)-6-O-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.
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