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Polymer Based Gene Silencing: In Vitro Delivery of SiRNA

Carlos, MI; Schätzlein, A; Uchegbu, I; (2016) Polymer Based Gene Silencing: In Vitro Delivery of SiRNA. In: Candiani, G, (ed.) Non-Viral Gene Delivery Vectors: Methods and Protocols: Part I. (pp. 149-157). Springer New York: New York, NY, USA.

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Abstract

Gene silencing may be achieved by harnessing the RNA interference mechanism to effect down-regulation of protein expression. The therapeutic use of siRNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA by N-(2-ethylamino)-6-O-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.

Type: Book chapter
Title: Polymer Based Gene Silencing: In Vitro Delivery of SiRNA
Location: United States
ISBN-13: 9781493937165
DOI: 10.1007/978-1-4939-3718-9_9
Publisher version: http://dx.doi.org/10.1007/978-1-4939-3718-9_9
Language: English
Keywords: Cationic polymer, Down-regulation, Gene delivery, N-(2-ethylamino)-6-O-glycolchitosan (EAGC), Transfection, Western Blotting, siRNA
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/1506356
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