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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Storm, MP; Sorrell, I; Shipley, R; Regan, S; Luetchford, KA; Sathish, J; Webb, S; (2016) Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture. Journal of Visualized Experiments (111) , Article e53431. 10.3791/53431. Green open access

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Abstract

Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture.

Type: Article
Title: Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
Open access status: An open access version is available from UCL Discovery
DOI: 10.3791/53431
Publisher version: http://dx.doi.org/10.3791/53431
Language: English
Additional information: Copyright © The Author(s), 2016. Copyright © 2016 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Further details about CC BY licenses are available at http://creativecommons.org/ licenses/by/3.0
Keywords: Bioengineering, Hollow fiber bioreactor, tissue culture, cell culture, 3D cell culture, hepatocyte, HepG2/C3A, albumin secretion
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Mechanical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1505814
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