Khanal, D;
Kondyurin, A;
Hau, H;
Knowles, JC;
Levinson, O;
Ramzan, I;
Fu, D;
... Chrzanowski, W; + view all
(2016)
Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study.
Analytical Chemistry
, 88
(15)
pp. 7530-7538.
10.1021/acs.analchem.6b00665.
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Abstract
The toxicity of nanomaterials raises major concerns because of the impact that nanomaterials may have on health, which remains poorly understood. We need to explore the fate of individual nanoparticles in cells at nano and molecular levels to establish their safety. Conformational changes in secondary protein structures are one of the main indicators of impaired biological function and hence, the ability to identify these changes at a nanoscale level offers unique insights into the nanotoxicity of materials. Here, we used nanoscale infrared spectroscopy and demonstrated for the first time that nanodiamonds induced alterations in both extra- and intracellular secondary protein structures, leading to the formation of antiparallel β-sheet, β-turns, intermolecular β- sheet and aggregation of proteins. These conformational changes of the protein structure may result in the loss of functionality of proteins and in turn lead to adverse effects.
| Type: | Article |
|---|---|
| Title: | Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1021/acs.analchem.6b00665 |
| Publisher version: | http://doi.org/10.1021/acs.analchem.6b00665 |
| Language: | English |
| Additional information: | Copyright © 2017 American Chemical Society. All rights reserved. This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://doi.org/10.1021/acs.analchem.6b00665 |
| Keywords: | Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, Atomic-force Microscope, Drug-delivery, Mammalian-cells, Cellular Uptake, In-vitro, Nanoparticles, Fibronectin, Adsorption, Diamond, Spectroscopy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1505801 |
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