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Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study

Khanal, D; Kondyurin, A; Hau, H; Knowles, JC; Levinson, O; Ramzan, I; Fu, D; ... Chrzanowski, W; + view all (2016) Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study. Analytical Chemistry , 88 (15) pp. 7530-7538. 10.1021/acs.analchem.6b00665. Green open access

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Abstract

The toxicity of nanomaterials raises major concerns because of the impact that nanomaterials may have on health, which remains poorly understood. We need to explore the fate of individual nanoparticles in cells at nano and molecular levels to establish their safety. Conformational changes in secondary protein structures are one of the main indicators of impaired biological function and hence, the ability to identify these changes at a nanoscale level offers unique insights into the nanotoxicity of materials. Here, we used nanoscale infrared spectroscopy and demonstrated for the first time that nanodiamonds induced alterations in both extra- and intracellular secondary protein structures, leading to the formation of antiparallel β-sheet, β-turns, intermolecular β- sheet and aggregation of proteins. These conformational changes of the protein structure may result in the loss of functionality of proteins and in turn lead to adverse effects.

Type: Article
Title: Biospectroscopy of Nanodiamond-Induced Alterations in Conformation of Intra- and Extracellular Proteins: A Nanoscale IR Study
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/acs.analchem.6b00665
Publisher version: http://doi.org/10.1021/acs.analchem.6b00665
Language: English
Additional information: Copyright © 2017 American Chemical Society. All rights reserved. This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://doi.org/10.1021/acs.analchem.6b00665
Keywords: Science & Technology, Physical Sciences, Chemistry, Analytical, Chemistry, Atomic-force Microscope, Drug-delivery, Mammalian-cells, Cellular Uptake, In-vitro, Nanoparticles, Fibronectin, Adsorption, Diamond, Spectroscopy
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1505801
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