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Mapping liver fat female-dependent quantitative trait loci in collaborative cross mice

Atamni, HJ; Botzman, M; Mott, R; Gat-Viks, I; Iraqi, FA; (2016) Mapping liver fat female-dependent quantitative trait loci in collaborative cross mice. Mammalian Genome , 27 (11) pp. 565-573. 10.1007/s00335-016-9658-3. Green open access

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Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease in the western world, with spectrum from simple steatosis to non-alcoholic steatohepatitis, which can progress to cirrhosis. NAFLD developments are known to be affected by host genetic background. Herein we emphasize the power of collaborative cross (CC) mouse for dissecting this complex trait and revealing quantitative trait loci (QTL) controlling hepatic fat accumulation in mice. 168 female and 338 male mice from 24 and 37 CC lines, respectively, of 18-20 weeks old, maintained on standard rodent diet, since weaning. Hepatic fat content was assessed, using dual DEXA scan in the liver. Using the available high-density genotype markers of the CC line, QTL mapping associated with percentage liver fat accumulation was performed. Our results revealed significant fatty liver accumulation QTL that were specifically, mapped in females. Two significant QTLs on chromosomes 17 and 18, with genomic intervals 3 and 2 Mb, respectively, were mapped. A third QTL, with a less significant P value, was mapped to chromosome 4, with genomic interval of 2 Mb. These QTLs were named Flal1-Flal3, referring to Fatty Liver Accumulation Locus 1-3, for the QTLs on chromosomes 17, 18, and 4, respectively. Unfortunately, no QTL was mapped with males. Searching the mouse genome database suggested several candidate genes involved in hepatic fat accumulation. Our results show that susceptibility to hepatic fat accumulations is a complex trait, controlled by multiple genetic factors in female mice, but not in male.

Type: Article
Title: Mapping liver fat female-dependent quantitative trait loci in collaborative cross mice
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00335-016-9658-3
Publisher version: http://doi.org/10.1007/s00335-016-9658-3
Language: English
Additional information: The final publication is available at Springer via http://dx.doi.org/10.1007/s00335-016-9658-3.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/1504013
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