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In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart

Ramesh, S; Singh, A; Cibi, DM; Hausenloy, DJ; Singh, MK; (2016) In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart. Journal of Visualized Experiments , 110 , Article e53993. 10.3791/53993. Green open access

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Abstract

During embryogenesis, the epicardial contribution to coronary vasculature development has been very well established. Cells derived from the epicardium differentiate into smooth muscle cells, fibroblasts and endothelial cells that contribute to the formation of coronary vessels. Here we have established an in vitro culture method for embryonic epicardial cells. Using genetic labelling, we have demonstrated that the majority of the migrating cells in our explant culture are of epicardial origin. Epicardial explant cells also retain the expression of epicardial markers (Wt1 and Tbx18). Furthermore, we provide evidence that epicardial explant cells undergo epithelial to mesenchymal transition (EMT), migrate and differentiate into smooth muscle cells after Transforming growth factor beta 1 (TGF-β1) treatment in a manner indistinguishable from that of epicardial cells in vivo. In conclusion, we provide a novel method for the culture of embryonic epicardial cells, which will help to explore the role of specific genes in epicardial cell biology.

Type: Article
Title: In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.3791/53993
Publisher version: http://dx.doi.org/10.3791/53993
Language: English
Additional information: Copyright © 2016 Journal of Visualized Experiments. The published version of record and related material is available at http://dx.doi.org/10.3791/53993
Keywords: Engineering, Epicardium, Proepicardium, Epithelial to mesenchymal transition (EMT), Coronary vasculature, Cardiovascular System, Cardiovascular Diseases
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Cardiovascular Science > Pre-clinical and Fundamental Science
URI: https://discovery.ucl.ac.uk/id/eprint/1496363
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