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Small cargoes pass through synthetically glued Golgi stacks

Dancourt, J; Zheng, H; Bottanelli, F; Allgeyer, ES; Bewersdorf, J; Graham, M; Liu, X; ... Lavieu, G; + view all (2016) Small cargoes pass through synthetically glued Golgi stacks. FEBS Letters , 590 (12) pp. 1675-1686. 10.1002/1873-3468.12210. Green open access

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Abstract

How are proteins transported across the stacked cisternae of the Golgi apparatus? Do they stay within the cisterna while the latter matures and progresses in an anterograde manner, or do they navigate between the cisternae via vesicles? Using synthetic biology, we engineered new tools designed to stabilize intercisternal adhesion such that Golgi cisternae are literally glued together, thus preventing any possible cisternal progression. Using bulk secretory assays and single-cell live imaging, we observed that small cargoes (but not large aggregated cargoes including collagen) still transited through glued Golgi, although the rate of transport was moderately reduced. ARF1, whose membrane recruitment is required for budding COPI vesicles, continues to cycle on and off glued Golgi. Numerous COPI-size vesicles were intercalated among the glued Golgi cisternae. These results suggest that cisternal progression is not required for anterograde transport, but do not address the possibility of cisternal maturation in situ.

Type: Article
Title: Small cargoes pass through synthetically glued Golgi stacks
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/1873-3468.12210
Publisher version: http://doi.org/10.1002/1873-3468.12210
Language: English
Additional information: © 2016 Federation of European Biochemical Societies. This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Cell Biology, Golgi, Membrane Trafficking, COILED-COIL PROTEINS, CISTERNAL MATURATION, ENDOPLASMIC-RETICULUM, TRANSPORT, DOMAIN, CELL, YEAST
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Experimental Epilepsy
URI: https://discovery.ucl.ac.uk/id/eprint/1496280
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