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In vivo confinement promotes collective migration of neural crest cells

Szabó, A; Melchionda, M; Nastasi, G; Woods, ML; Campo, S; Perris, R; Mayor, R; (2016) In vivo confinement promotes collective migration of neural crest cells. Journal of Cell Biology , 213 (5) pp. 543-555. 10.1083/jcb.201602083. Green open access

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Abstract

Collective cell migration is fundamental throughout development and in many diseases. Spatial confinement using micropatterns has been shown to promote collective cell migration in vitro, but its effect in vivo remains unclear. Combining computational and experimental approaches, we show that the in vivo collective migration of neural crest cells (NCCs) depends on such confinement. We demonstrate that confinement may be imposed by the spatiotemporal distribution of a nonpermissive substrate provided by versican, an extracellular matrix molecule previously proposed to have contrasting roles: barrier or promoter of NCC migration. We resolve the controversy by demonstrating that versican works as an inhibitor of NCC migration and also acts as a guiding cue by forming exclusionary boundaries. Our model predicts an optimal number of cells in a given confinement width to allow for directional migration. This optimum coincides with the width of neural crest migratory streams analyzed across different species, proposing an explanation for the highly conserved nature of NCC streams during development.

Type: Article
Title: In vivo confinement promotes collective migration of neural crest cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1083/jcb.201602083
Publisher version: http://dx.doi.org/10.1083/jcb.201602083
Language: English
Additional information: Copyright © 2016 Szabó et al. Published by The Rockefeller University Press and available at http://dx.doi.org/10.1083/jcb.201602083. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1496071
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