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Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

Franzin, C; Piccoli, M; Urbani, L; Biz, C; Gamba, P; De Coppi, P; Pozzobon, M; (2016) Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies. In: Turksen, K, (ed.) Stem Cell Heterogeneity: Methods and Protocols. (pp. 195-204). Springer New York: New York. Green open access

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Abstract

One of the major issues concerning human skeletal muscle progenitor cells is represented by the efficient isolation and in vitro expansion of cells retaining the ability to proliferate, migrate and differentiate once transplanted. Here we describe a method (1) effective in obtaining human muscle precursor cells both from fresh and frozen biopsies coming from different muscles, (2) selective to yield cells uniformly positive for CD56 and negative for CD34 without FACS sorting, (3) reliable in maintaining proliferative and in vitro differentiative capacity up to passage 10.

Type: Book chapter
Title: Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies
Location: United States
ISBN-13: 9781493965496
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/7651_2016_321
Publisher version: http://dx.doi.org/10.1007/7651_2016_321
Language: English
Additional information: Copyright © 2016 Springer Science+Business Media New York. The final publication is available at Springer via http://dx.doi.org/10.1007/7651_2016_321.
Keywords: CD56, Enzymatic digestion, Human muscle cell primary culture, Human muscle precursor cells, Skeletal muscle biopsy
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1494291
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