Szoboszlay, M;
Lorincz, A;
Lanore, F;
Vervaeke, K;
Silver, RA;
Nusser, Z;
(2016)
Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells.
Neuron
, 90
(5)
pp. 1043-1056.
10.1016/j.neuron.2016.03.029.
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Abstract
The strength and variability of electrical synaptic connections between GABAergic interneurons are key determinants of spike synchrony within neuronal networks. However, little is known about how electrical coupling strength is determined due to the inaccessibility of gap junctions on the dendritic tree. We investigated the properties of gap junctions in cerebellar interneurons by combining paired somato-somatic and somato-dendritic recordings, anatomical reconstructions, immunohistochemistry, electron microscopy, and modeling. By fitting detailed compartmental models of Golgi cells to their somato-dendritic voltage responses, we determined their passive electrical properties and the mean gap junction conductance (0.9 nS). Connexin36 immunofluorescence and freeze-fracture replica immunogold labeling revealed a large variability in gap junction size and that only 18% of the 340 channels are open in each plaque. Our results establish that the number of gap junctions per connection is the main determinant of both the strength and variability in electrical coupling between Golgi cells.
| Type: | Article |
|---|---|
| Title: | Functional Properties of Dendritic Gap Junctions in Cerebellar Golgi Cells |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.neuron.2016.03.029 |
| Publisher version: | http://dx.doi.org/10.1016/j.neuron.2016.03.029 |
| Language: | English |
| Additional information: | © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Neurosciences, Neurosciences & Neurology, COUPLED INTERNEURON NETWORK, PYRAMIDAL NEURON DENDRITES, PH-DEPENDENT MODULATION, GOLDFISH MAUTHNER CELL, PATCH-CLAMP RECORDINGS, LONG-TERM MODULATION, ELECTRICAL SYNAPSES, GABAERGIC INTERNEURONS, SYNAPTIC INTEGRATION, INHIBITORY INTERNEURONS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1494139 |
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