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Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by DACT1/2

Rabadán, MA; Herrera, A; Fanlo, L; Usieto, S; Carmona-Fontaine, C; Barriga, EH; Mayor, R; ... Martí, E; + view all (2016) Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by DACT1/2. Development , 143 (12) pp. 2194-2205. 10.1242/dev.134981. Green open access

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Abstract

Delamination of neural crest (NC) cells is a bona fide physiological model of epithelial-to-mesenchymal transition (EMT), a process that is influenced by Wnt/β-catenin signalling. Using two in vivo models, we show that Wnt/β-catenin signalling is transiently inhibited at the time of NC delamination. In attempting to define the mechanism underlying this inhibition, we found that the scaffold proteins Dact1 and Dact2, which are expressed in pre-migratory NC cells, are required for NC delamination in Xenopus and chick embryos, whereas they do not affect the motile properties of migratory NC cells. Dact1/2 inhibit Wnt/β-catenin signalling upstream of the transcriptional activity of T cell factor (TCF), which is required for EMT to proceed. Dact1/2 regulate the subcellular distribution of β-catenin, preventing β-catenin from acting as a transcriptional co-activator to TCF, yet without affecting its stability. Together, these data identify a novel yet important regulatory element that inhibits β-catenin signalling, which then affects NC delamination.

Type: Article
Title: Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by DACT1/2
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.134981
Publisher version: http://dx.doi.org/10.1242/dev.134981
Language: English
Additional information: Copyright © 2016 The author. Published by The Company of Biologists Ltd. This article (Rabadán, MA et al (2016) Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by DACT1/2. Development, 143 (12) pp. 2194-2205. 10.1242/dev.134981) is made available in accordance with the publisher's self-archiving policy and available at http://dev.biologists.org/content/143/12/2194
Keywords: Dishevelled antagonist of b-Catenin; Dapper; Frodo; b-Catenin; nuclear bodies; Xenopus embryo; chick embryo
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1489779
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