Gunnell, A;
Webb, HM;
Wood, CD;
McClellan, MJ;
Wichaidit, B;
Kempkes, B;
Jenner, RG;
... West, MJ; + view all
(2016)
RUNX super-enhancer control through the Notch pathway by Epstein-Barr virus transcription factors regulates B cell growth.
Nucleic Acids Research
, 44
(10)
, Article 4636-4650. 10.1093/nar/gkw085.
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RUNX super-enhancer control through the Notch pathway by Epstein-Barr virus transcription factors regulates B cell growth.pdf - Published Version Download (2MB) | Preview |
Abstract
In B cells infected by the cancer-associated Epstein-Barr virus (EBV), RUNX3 and RUNX1 transcription is manipulated to control cell growth. The EBV-encoded EBNA2 transcription factor (TF) activates RUNX3 transcription leading to RUNX3-mediated repression of the RUNX1 promoter and the relief of RUNX1-directed growth repression. We show that EBNA2 activates RUNX3 through a specific element within a −97 kb super-enhancer in a manner dependent on the expression of the Notch DNA-binding partner RBP-J. We also reveal that the EBV TFs EBNA3B and EBNA3C contribute to RUNX3 activation in EBV-infected cells by targeting the same element. Uncovering a counter-regulatory feed-forward step, we demonstrate EBNA2 activation of a RUNX1 super-enhancer (−139 to −250 kb) that results in low-level RUNX1 expression in cells refractory to RUNX1-mediated growth inhibition. EBNA2 activation of the RUNX1 super-enhancer is also dependent on RBP-J. Consistent with the context-dependent roles of EBNA3B and EBNA3C as activators or repressors, we find that these proteins negatively regulate the RUNX1 super-enhancer, curbing EBNA2 activation. Taken together our results reveal cell-type-specific exploitation of RUNX gene super-enhancers by multiple EBV TFs via the Notch pathway to fine tune RUNX3 and RUNX1 expression and manipulate B-cell growth.
| Type: | Article |
|---|---|
| Title: | RUNX super-enhancer control through the Notch pathway by Epstein-Barr virus transcription factors regulates B cell growth |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1093/nar/gkw085 |
| Publisher version: | http://dx.doi.org/10.1093/nar/gkw085 |
| Additional information: | © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, RBP-J-KAPPA, SIGNAL BINDING-PROTEIN, NUCLEAR ANTIGEN-2, BURKITTS-LYMPHOMA, DOWN-REGULATION, 3C BINDS, C-MYC, EBNA-2, DOMAIN, DNA |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1489497 |
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