Juenemann, AGM;
Rejdak, R;
Huchzermeyer, C;
Maciejewski, R;
Grieb, P;
Kruse, FE;
Zrenner, E;
... Petzold, A; + view all
(2015)
Elevated vitreous body glial fibrillary acidic protein in retinal diseases.
Graefe's Archive for Clinical and Experimental Ophthalmology
, 253
(12)
pp. 2181-2186.
10.1007/s00417-015-3127-7.
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Abstract
Purpose: Increased expression of glial fibrillary acidic protein (GFAP) is a characteristic of gliotic activation (Müller cells and astrocytes) in the retina. This study assessed vitreous body GFAP levels in various forms of retinal pathology. Methods: This prospective study included 82 patients who underwent vitrectomy (46 retinal detachments (RDs), 13 macular hole (MHs), 15 epiretinal glioses (EGs), 8 organ donors). An established enzyme–linked immunosorbent assay (ELISA, SMI26) was used for quantification of GFAP. Results: The highest concentration of vitreous body GFAP in organ donors was 20 pg/mL and it was used as the cutoff. A significant proportion of patients suffering from RD (65 %) to EG (53 %) had vitreous body GFAP levels above this cutoff when compared to organ donors (0 %, p < 0.0001, p = 0.0194, respectively, Fisher’s exact test) and MH (8 %, p < 0.0001, p = 0.0157, respectively). In RD and EG, vitreous body GFAP levels were correlated with axial length (R = 0.69, R = 0.52, p < 0.05 for both). Conclusions: The data suggest that human vitreous body GFAP is a protein biomarker for glial activation in response to retinal pathologies. Vitreous body GFAP levels may be of interest as a surrogate outcome for experimental treatment strategies in translational studies.
| Type: | Article |
|---|---|
| Title: | Elevated vitreous body glial fibrillary acidic protein in retinal diseases |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/s00417-015-3127-7 |
| Publisher version: | http://doi.org/10.1007/s00417-015-3127-7 |
| Language: | English |
| Additional information: | Copyright © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
| Keywords: | Vitreous . Glial fibrillary acidic protein (GFAP) . Biomarker . Retinal detachment . Epiretinal gliosis . Müller cells |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1489036 |
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