Kunda, NK;
Alfagih, IM;
Miyaji, EN;
Figueiredo, DB;
Goncalves, VM;
Ferreira, DM;
Dennison, SR;
... Saleem, IY; + view all
(2015)
Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles.
International Journal of Pharmaceutics
, 495
(2)
pp. 903-912.
10.1016/j.ijpharm.2015.09.034.
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Abstract
Pneumonia, caused by Streptococcus pneumoniae, mainly affects the immunocompromised, the very young and the old, and remains one of the leading causes of death. A steady rise in disease numbers from non-vaccine serotypes necessitates a new vaccine formulation that ideally has better antigen stability and integrity, does not require cold-chain and can be delivered non-invasively. In this study, a dry powder vaccine containing an important antigen of S. pneumoniae, pneumococcal surface protein A (PspA) that has shown cross-reactivity amongst serotypes to be delivered via the pulmonary route has been formulated. The formulation contains the antigen PspA adsorbed onto the surface of polymeric nanoparticles encapsulated in l-leucine microparticles that can be loaded into capsules and delivered via an inhaler. We have successfully synthesized particles of ∼150 nm and achieved ∼20 μg of PspA adsorption per mg of NPs. In addition, the spray-dried powders displayed a FPF of 74.31 ± 1.32% and MMAD of 1.70 ± 0.03 μm suggesting a broncho-alveolar lung deposition facilitating the uptake of the nanoparticles by dendritic cells. Also, the PspA released from the dry powders maintained antigen stability (SDS-PAGE), integrity (Circular dichroism) and activity (lactoferrin binding assay). Moreover, the released antigen also maintained its antigenicity as determined by ELISA.
| Type: | Article |
|---|---|
| Title: | Pulmonary dry powder vaccine of pneumococcal antigen loaded nanoparticles |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijpharm.2015.09.034 |
| Publisher version: | http://dx.doi.org/ 10.1016/j.ijpharm.2015.09.034 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript/version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Nanoparticle; Dry powder inhalation; Pneumococcal surface protein A (PspA); Nanocomposite microcarrier; Dendritic cells; Antigen delivery system |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1484023 |
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