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Integrated economic and experimental framework for screening of primary recovery technologies for high cell density CHO cultures

Popova, D; Stonier, A; Pain, D; Titchener-Hooker, NJ; Farid, SS; (2016) Integrated economic and experimental framework for screening of primary recovery technologies for high cell density CHO cultures. Biotechnology Journal , 11 (7) pp. 899-909. 10.1002/biot.201500336. Green open access

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Abstract

Increases in mammalian cell culture titres and densities have placed significant demands on primary recovery operation performance. This article presents a methodology which aims to screen rapidly and evaluate primary recovery technologies for their scope for technically feasible and cost-effective operation in the context of high cell density mammalian cell cultures. It was applied to assess the performance of current (centrifugation and depth filtration options) and alternative (tangential flow filtration (TFF)) primary recovery strategies. Cell culture test materials (CCTM) were generated to simulate the most demanding cell culture conditions selected as a screening challenge for the technologies. The performance of these technology options was assessed using lab scale and ultra scale-down (USD) mimics requiring 25-110mL volumes for centrifugation and depth filtration and TFF screening experiments respectively. A centrifugation and depth filtration combination as well as both of the alternative technologies met the performance selection criteria. A detailed process economics evaluation was carried out at three scales of manufacturing (2,000L, 10,000L, 20,000L), where alternative primary recovery options were shown to potentially provide a more cost-effective primary recovery process in the future. This assessment process and the study results can aid technology selection to identify the most effective option for a specific scenario.

Type: Article
Title: Integrated economic and experimental framework for screening of primary recovery technologies for high cell density CHO cultures
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/biot.201500336
Publisher version: http://dx.doi.org/10.1002/biot.201500336
Language: English
Additional information: © 2016 The Authors. Biotechnology Journal published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Bioseparation, High cell density, Mammalian cell culture, Primary recovery, Tangential flow filtration
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1482781
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