New, SP;
Ibrahim, A;
Guasti, L;
Zucchelli, E;
Birchall, M;
Bulstrode, NW;
Seifalian, AM;
(2017)
Towards reconstruction of epithelialized cartilages from autologous adipose tissue-derived stem cells.
Journal of Tissue Engineering and Regenerative Medicine
, 11
(11)
pp. 3078-3089.
10.1002/term.2211.
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Abstract
Deformities of the upper airways, including those of the nose and throat, are typically corrected by reconstructive surgery. The use of autologous somatic stem cells for repair of defects could improve quality and outcomes of such operations. We explored the ability of paediatric adipose-derived stem cells (pADSCs), a readily available source of autologous stem cells, to generate a cartilage construct with a functional epithelium. pADSCs seeded on the biodegradable nanocomposite polymer, POSS-PCL, proliferated and differentiated towards mesenchymal lineages. ADSCs infiltrated 3D POSS-PCL nanoscaffold and chondroid matrix was observed throughout chondrogenically-induced samples. In ovo chorioallantoic membrane-grafted ADSC-nanoscaffold composites were enwrapped by host vessels indicating good compatibility in an in vivo system. Furthermore, pADSCs could be induced to transdifferentiate towards barrier-forming epithelial-like cells. By combining differentiation protocols, we were able to generate epithelial cell lined chondrogenic micromasses from the same pADSC line. This proof-of-concept study is the first to our knowledge to demonstrate that individual pADSC lines can differentiate towards two different germlines and be successfully co-cultured. This has important implications for bioengineering of paediatric airways and further confirms the plastic nature of ADSCs.
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