UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment

Sellar, RS; Fraser, L; Khwaja, A; Gale, RE; Marafioti, T; Akarca, A; Hubank, M; ... Linch, DC; + view all (2016) Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment. British Journal of Haematology , 174 (2) pp. 275-279. 10.1111/bjh.14055. Green open access

[img]
Preview
Text
Gale_Sellar_BJH 2015-01938 -revised.pdf

Download (475kB) | Preview

Abstract

Using immunohistochemistry and flow cytometry to define phases of the cell cycle, this study shows that a high proportion of acute myeloid leukaemia (AML) blasts obtained from trephine biopsies are cycling, whereas >95% of peripheral blood-derived blasts are arrested in G1 . Results obtained from bone marrow aspirates are more similar to those from blood rather than from trephine biopsies. These differences were confirmed by gene expression profiling in a patient with high count AML. This has implications for cell cycle and other biological studies using aspirates rather than trephine biopsies and for the use of cell mobilising agents before chemotherapy.

Type: Article
Title: Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/bjh.14055
Publisher version: http://dx.doi.org/10.1111/bjh.14055
Language: English
Additional information: This is the peer reviewed version of the following article: Sellar, RS; Fraser, L; Khwaja, A; Gale, RE; Marafioti, T; Akarca, A; Hubank, M; (2016) Cell cycle status in AML blast cells from peripheral blood, bone marrow aspirates and trephines and implications for biological studies and treatment. British Journal of Haematology, which has been published in final form at http://dx.doi.org/10.1111/bjh.14055. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Keywords: AML, cell cycle, flow cytometry, gene expression, immunocytochemistry
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Haematology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Pathology
URI: https://discovery.ucl.ac.uk/id/eprint/1480901
Downloads since deposit
68Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item