Blanco-Andujar, C;
Ortega, D;
Southern, P;
Nesbitt, SA;
Thanh, NT;
Pankhurst, QA;
(2016)
Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia.
Nanomedicine
, 11
(2)
pp. 121-136.
10.2217/nnm.15.185.
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Abstract
AIM: To assess cell death pathways in response to magnetic hyperthermia. MATERIALS & METHODS: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. RESULTS: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. CONCLUSION: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
| Type: | Article |
|---|---|
| Title: | Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.2217/nnm.15.185 |
| Publisher version: | http://dx.doi.org/10.2217/nnm.15.185 |
| Additional information: | © Nguyễn Thị Kim Thanh. This work is licensed under a Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | apoptosis, cell death pathways, human melanoma cells, magnetic hyperthermia, magnetic nanoparticles |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1479393 |
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