UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A comparative study of the in vitro permeation of ibuprofen in mammalian skin, the PAMPA model and silicone membrane

Lane, M; Hadgraft, J; Patel, A; Wibawa, J; Bell, M; Sinko, B; (2016) A comparative study of the in vitro permeation of ibuprofen in mammalian skin, the PAMPA model and silicone membrane. International Journal of Pharmaceutics , 505 (1-2) pp. 14-19. 10.1016/j.ijpharm.2016.03.043. Green open access

[thumbnail of MANUSCRIPTREVISION.pdf]
Preview
Text
MANUSCRIPTREVISION.pdf - Accepted Version

Download (557kB) | Preview

Abstract

Human skin remains the membrane of choice when conducting in vitro studies to determine dermal penetration of active pharmaceutical ingredients or xenobiotics. However there are ethical and safety issues associated with obtaining human tissue. For these reasons synthetic membranes, cell culture models or in silico predictive algorithms have been researched intensively as alternative approaches to predict dermal exposure in man. Porcine skin has also been recommended as an acceptable surrogate for topical or transdermal delivery research. Here we examine the in vitro permeation of a model active, ibuprofen, using human or porcine skin, as well as the Parallel Artificial Membrane Permeation Assay (PAMPA) model and silicone membrane. Finite dose studies were conducted in all models using commercial ibuprofen formulations and simple volatile ibuprofen solutions. The dose applied in the PAMPA model was also varied in order to determine the amount of applied formulation which best simulates typical amounts of topical products applied by patients or consumers. Permeation studies were conducted up to 6 h for PAMPA and silicone and up to 48 h for human and porcine skin. Cumulative amounts permeated at 6 h were comparable for PAMPA and silicone, ranging from 91–136 g/cm2 across the range of formulations studied. At 48 h, maximum ibuprofen permeation in human skin ranged from 11–38 g/cm2 and corresponding values in porcine skin were 59–81 g/cm2. A dose of 1 l/cm2 was confirmed as appropriate for finite dose studies in the PAMPA model. The formulation which delivered the greatest amount of ibuprofen in human skin was also significantly more efficient than other formulations when evaluated in the PAMPA model. The PAMPA model also discriminated between different formulation types (i.e. gel versus solution) compared with other models. Overall, the results confirm the more permeable nature of the PAMPA, silicone membrane and porcine tissue models to ibuprofen compared with human skin. Further finite dose studies to elucidate the effects of individual excipients on the barrier properties of the PAMPA model are needed to expand the applications of this model. The range of actives that are suitable for study using the model also needs to be delineated.

Type: Article
Title: A comparative study of the in vitro permeation of ibuprofen in mammalian skin, the PAMPA model and silicone membrane
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ijpharm.2016.03.043
Publisher version: http://dx.doi.org/10.1016/j.ijpharm.2016.03.043
Language: English
Additional information: © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0.
Keywords: Human skin, porcine skin, silicone, PAMPA, ibuprofen, permeation
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/1477393
Downloads since deposit
678Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item