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Synthesis, characterization, and biocompatible properties of alanine-grafted chitosan copolymers

Park, GH; Kang, MS; Knowles, JC; Gong, MS; (2016) Synthesis, characterization, and biocompatible properties of alanine-grafted chitosan copolymers. Journal of Biomaterials Applications , 30 (9) pp. 1350-1361. 10.1177/0885328215626892. Green open access

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Abstract

In order to overcome major problems regarding the lack of affinity to solvents and limited reactivity of the free amines of chitosan, introduction of appropriate spacer arms having terminal amine function is considered of interest. L-Alanine-N-carboxyanhydride was grafted onto chitosan via anionic ring-opening polymerization. The chemical and structural characterizations of L-alanine-grafted chitosan (Ala-g-Cts) were confirmed through Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy ((1)H NMR). In addition, the viscoelastic properties of Ala-g-Cts were examined by means of a rotational viscometer, and thermal analysis was carried out with a thermogravimetric analyzer and differential scanning calorimetry. Morphological changes in the chitosan L-alanine moiety were determined by x-ray diffraction. To determine the feasibility of using these films as biomedical materials, we investigated the effects of their L-alanine content on physical and mechanical properties. The biodegradation results of crosslinked Ala-g-Cts films were evaluated in phosphate-buffered solution containing lysozyme at 37℃. Proliferation of MC3T3-E1 cells on crosslinked Ala-g-Cts films was also investigated with use of the CCK-8 assay.

Type: Article
Title: Synthesis, characterization, and biocompatible properties of alanine-grafted chitosan copolymers
Open access status: An open access version is available from UCL Discovery
DOI: 10.1177/0885328215626892
Publisher version: http://dx.doi.org/10.1177/0885328215626892
Language: English
Additional information: © The Author(s) 2016. Reprints and permissions: sagepub.co.uk/journalsPermissions.nav
Keywords: Chitosan, L-alanine-NCA, MC3T3-E1 cells, biocompatibility, graft polymer
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1476121
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