UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS

McDonnell, T; Pericleous, C; Laurine, E; Tommasi, R; Garza-Garcia, A; Giles, I; Ioannou, Y; (2015) Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS. BMC Biotechnology , 15 , Article 104. 10.1186/s12896-015-0222-0. Green open access

[thumbnail of Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS.pdf]
Preview
Text
Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS.pdf - Published Version

Download (2MB) | Preview

Abstract

BACKGROUND: In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of β2GPI rendering this domain of interest as a possible therapeutic. RESULTS: This new method of production of Domain I of β2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50-75 mg of pure, folded, active Domain I of β2GPI per litre of expression media. CONCLUSION: The application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic.

Type: Article
Title: Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1186/s12896-015-0222-0
Publisher version: http://dx.doi.org/10.1186/s12896-015-0222-0
Language: English
Additional information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1475038
Downloads since deposit
93Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item