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The exon junction complex is required for definition and excision of neighboring introns in Drosophila

Hayashi, R; Handler, D; Ish-Horowicz, D; Brennecke, J; (2014) The exon junction complex is required for definition and excision of neighboring introns in Drosophila. Genes and Development , 28 (16) pp. 1772-1785. 10.1101/gad.245738.114. Green open access

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Abstract

Splicing of pre-mRNAs results in the deposition of the exon junction complex (EJC) upstream of exon-exon boundaries. The EJC plays crucial post-splicing roles in export, translation, localization, and nonsense-mediated decay of mRNAs. It also aids faithful splicing of pre-mRNAs containing large introns, albeit via an unknown mechanism. Here, we show that the core EJC plus the accessory factors RnpS1 and Acinus aid in definition and efficient splicing of neighboring introns. This requires prior deposition of the EJC in close proximity to either an upstream or downstream splicing event. If present in isolation, EJC-dependent introns are splicing-defective also in wild-type cells. Interestingly, the most affected intron belongs to the piwi locus, which explains the reported transposon desilencing in EJC-depleted Drosophila ovaries. Based on a transcriptome-wide analysis, we propose that the dependency of splicing on the EJC is exploited as a means to control the temporal order of splicing events.

Type: Article
Title: The exon junction complex is required for definition and excision of neighboring introns in Drosophila
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1101/gad.245738.114
Publisher version: http://dx.doi.org/10.1101/gad.245738.114
Language: English
Additional information: Copyright © 2014 Hayashi et al.; Published by Cold Spring Harbor Laboratory Press. This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.
Keywords: Piwi–piRNA pathway, SR proteins Acinus and RnpS1, exon junction complex, intron definition, splicing, transposon silencing, Animals, Argonaute Proteins, Cells, Cultured, DNA Transposable Elements, Drosophila, Drosophila Proteins, Female, Gene Expression Regulation, Gene Silencing, Introns, Ovary, RNA Precursors, RNA Splicing
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1474832
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