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Patient-specific hiPSC bioprocessing for drug screening: Bioprocess economics and optimisation

Jenkins, M; Bilsland, J; Allsopp, TE; Ho, SV; Farid, SS; (2016) Patient-specific hiPSC bioprocessing for drug screening: Bioprocess economics and optimisation. Biochemical Engineering Journal , 108 pp. 84-97. 10.1016/j.bej.2015.09.024. Green open access

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Abstract

This paper describes a decisional tool that is designed to identify cost-effective process designs for drug screening products derived from human induced pluripotent stem cells (hiPSC). The decisional tool comprises a bioprocess economics model linked to a search algorithm to assess the financial impact of manual and automated bioprocessing strategies that use 2D-planar tissue culture technologies. The tool was applied to a case study that examines the production of patient-specific iPSC-derived neurons for drug screening. The production strategies were compared across three analytical drug screening methods, each requiring cell production at a distinct scale (manual patch-clamp analysis, high throughput screening and plate-based pharmacology), as well as different annual cell line utilization requirements ('throughputs') (between 10 and 100 lines) so as to represent different industry scenarios. The tool determined the critical cell line throughput where the most cost-effective production strategy switched from the manual to automated workflow. The key process economics driver was the number of iPSC expansion stages required. Stochastic modelling of the bioprocess illustrated that the automated was more robust than the manual workflow in the scenarios investigated. The tool predicted the level of performance improvements required in iPSC expansion and differentiation as well as reductions in indirect costs and media costs so as to achieve an acceptable cost of goods (COG).

Type: Article
Title: Patient-specific hiPSC bioprocessing for drug screening: Bioprocess economics and optimisation
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bej.2015.09.024
Publisher version: http://dx.doi.org/10.1016/j.bej.2015.09.024
Language: English
Additional information: Copyright © 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Human induced pluripotent stem cells, Drug screening, Modelling, Optimisation, Bioprocess design, Scale up
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1474210
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