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Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring

Falkenberg, LJ; Havenhand, JN; Styan, CS; (2016) Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring. Marine Environmental Research , 114 pp. 51-57. 10.1016/j.marenvres.2015.12.005. Green open access

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Abstract

Forecasting the impacts of changes in water quality on broadcast spawning aquatic organisms is a key aspect of environmental monitoring. Rapid assays of reproductive potential are central to this monitoring, and there is a need to develop a variety of methods to identify responses. Here, we report a proof-of-concept study that assesses whether quantification of “Sperm Accumulated Against Surface” (SAAS) of tissue culture well-plates could be a rapid and simple proxy measure of fertilisation success. Our results confirm that motile sperm (but not immotile sperm) actively accumulate at surfaces and that the pattern of accumulation reflects fertilisation success in the model oyster species Crassostrea gigas. Furthermore, we confirm these patterns of SAAS for another marine species, the polychaete Galeolaria caespitosa, as well as for a freshwater species, the fish Gasterosteus aculeatus. For all species considered, SAAS reflected changes in sperm performance caused by experimentally manipulated differences in water quality (here, salinity). These findings indicate that SAAS could be applied easily to a range of species when examining the effects of water quality. Measurement of SAAS could, therefore, form the basis of a rapid and reliable assay for bioassessments of broadcast spawning aquatic organisms.

Type: Article
Title: Sperm Accumulated Against Surface: A novel alternative bioassay for environmental monitoring
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.marenvres.2015.12.005
Publisher version: http://dx.doi.org/10.1016/j.marenvres.2015.12.005
Language: English
Additional information: © 2015 Elsevier Ltd. This manuscript version is made available under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International license (CC BY-NC-ND 4.0). This license allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licenses are available at http://creativecommons.org/ licenses/by/4.0. Access may be initially restricted by the publisher.
Keywords: Bioassay; Bioassessment; Ecotoxicology; Oyster; Polychaete; Sperm movement; Spermatozoa; Stickleback
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Engineering Science Faculty Office
URI: https://discovery.ucl.ac.uk/id/eprint/1473593
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