Ibrahim, SSM;
(2015)
Exposure and characterisation of collagen ultrastructure in primary teeth affected with Osteogenesis Imperfecta and Dentinogenesis Imperfecta.
Doctoral thesis , (UCL) University College London.
Preview |
Text
Ibrahim_Salwa_Thesis-2015_thesis_only.pdf Download (15MB) | Preview |
Abstract
Common genetic diseases associated with collagen abnormalities include Osteogenesis Imperfecta (OI) and Dentinogenesis Imperfecta (DI). Whilst the effects of collagen abnormalities in OI are fairly well-known in bone, there is limited information regarding their effects on dentine. This study aims to characterise the microstructure of dentinal collagen, using Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) in DI, OI and control primary teeth. The main aim of this study was first to develop a demineralisation protocol to systematically expose dentinal collagen whilst preventing its degradation. Then, topological assessment of the collagen scaffold at the nano scale level and comparison of characteristic morphological markers was done. Sample-specific demineralisation protocols were developed using a combination of 37% phosphoric acid and 6.5% Sodium Hypochlorite to expose the dentinal collagen. The protocol effectiveness was evaluated using Fourier Transform Spectroscopy (FTIR) and Raman spectroscopy. (AFM) and (SEM) images of exposed dentinal collagen were collected, analysed, and compared to identify specific ultrastructural markers. Dentine sections of 4 control, DI type I (DI associated with OI), and DI type II primary teeth were examined under ethical approval. Dentine in both DI type I and type II samples showed significantly less numbers of dentinal tubules with varying shape and size. Unlike control samples, irregular collagen mesh-network arrangements with inconsistent fibril diameter, local swelling, and abrupt discontinuity were noted. These observations may explain poor dentine quality and adhesion difficulties. In conclusion, it was hypothesised that the interpretation of results and the identification of specific OI/DI markers will guide us into better understanding of the nature and management of these rare conditions.
| Type: | Thesis (Doctoral) |
|---|---|
| Title: | Exposure and characterisation of collagen ultrastructure in primary teeth affected with Osteogenesis Imperfecta and Dentinogenesis Imperfecta |
| Event: | (UCL) University College London |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Keywords: | collagen, dentine, dentinogenesis imperfecta, osteogenesis imperfecta, DI, OI, dentinal collagen exposure, demineralisation protocol, demineralisation of dentine, dentine demineralisation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > EDI Craniofacial and Development Sci |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1473331 |
Archive Staff Only
 |
View Item |
