UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature

Cremona, A; Orsini, F; Corsetto, PA; Hoogenboom, BW; Rizzo, AM; (2015) Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature. PLoS One , 10 (7) , Article e0132696. 10.1371/journal.pone.0132696. Green open access

[thumbnail of Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature..pdf]
Preview
Text
Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature..pdf

Download (4MB) | Preview

Abstract

The formation of lipid microdomains ("rafts") is presumed to play an important role in various cellular functions, but their nature remains controversial. Here we report on microdomain formation in isolated, detergent-resistant membranes from MDA-MB-231 human breast cancer cells, studied by atomic force microscopy (AFM). Whereas microdomains were readily observed at room temperature, they shrunk in size and mostly disappeared at higher temperatures. This shrinking in microdomain size was accompanied by a gradual reduction of the height difference between the microdomains and the surrounding membrane, consistent with the behaviour expected for lipids that are laterally segregated in liquid ordered and liquid disordered domains. Immunolabeling experiments demonstrated that the microdomains contained flotillin-1, a protein associated with lipid rafts. The microdomains reversibly dissolved and reappeared, respectively, on heating to and cooling below temperatures around 37 °C, which is indicative of radical changes in local membrane order close to physiological temperature.

Type: Article
Title: Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0132696
Publisher version: http://dx.doi.org/10.1371/journal.pone.0132696
Language: English
Additional information: © 2015 Cremona et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/1472661
Downloads since deposit
72Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item