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The effect of Young's modulus on the neuronal differentiation of mouse embryonic stem cells

Ali, S; Wall, IB; Mason, C; Pelling, AE; Veraitch, FS; (2015) The effect of Young's modulus on the neuronal differentiation of mouse embryonic stem cells. Acta Biomaterialia , 25 pp. 253-267. 10.1016/j.actbio.2015.07.008. Green open access

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Abstract

There is substantial evidence that cells produce a diverse response to changes in ECM stiffness depending on their identity. Our aim was to understand how stiffness impacts neuronal differentiation of embryonic stem cells (ESC's), and how this varies at three specific stages of the differentiation process. In this investigation, three effects of stiffness on cells were considered; attachment, expansion and phenotypic changes during differentiation. Stiffness was varied from 2kPa to 18kPa to finally 35kPa. Attachment was found to decrease with increasing stiffness for both ESC's (with a 95% decrease on 35kPa compared to 2kPa) and neural precursors (with a 83% decrease on 35kPa). The attachment of immature neurons was unaffected by stiffness. Expansion was independent of stiffness for all cell types, implying that the proliferation of cells during this differentiation process was independent of Young's modulus. Stiffness had no effect upon phenotypic changes during differentiation for mESC's and neural precursors. 2kPa increased the proportion of cells that differentiated from immature into mature neurons. Taken together our findings imply that the impact of Young's modulus on attachment diminishes as neuronal cells become more mature. Conversely, the impact of Young's modulus on changes in phenotype increased as cells became more mature.

Type: Article
Title: The effect of Young's modulus on the neuronal differentiation of mouse embryonic stem cells
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.actbio.2015.07.008
Publisher version: http://dx.doi.org/10.1016/j.actbio.2015.07.008
Language: English
Additional information: © 2015 Acta Materialia Inc. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Atomic force microscopy, Embryonic stem cells, Neuronal differentiation, Young’s modulus
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1472560
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