UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range

Hazael, R; Foglia, F; Kardzhaliyska, L; Daniel, I; Meersman, F; McMillan, P; (2014) Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range. Frontiers in Microbiology , 5 10.3389/fmicb.2014.00612. Green open access

[thumbnail of Hazael et al. Shewanella oneidensis MR-1 .pdf]
Preview
Text
Hazael et al. Shewanella oneidensis MR-1 .pdf

Download (2MB) | Preview

Abstract

The survival of Shewanella oneidensis MR-1 at up to 1500 MPa was investigated by laboratory studies involving exposure to high pressure followed by evaluation of survivors as the number (N) of colony forming units (CFU) that could be cultured following recovery to ambient conditions. Exposing the wild type (WT) bacteria to 250 MPa resulted in only a minor (0.7 log N units) drop in survival compared with the initial concentration of 108 cells/ml. Raising the pressure to above 500 MPa caused a large reduction in the number of viable cells observed following recovery to ambient pressure. Additional pressure increase caused a further decrease in survivability, with approximately 102 CFU/ml recorded following exposure to 1000 MPa (1 GPa) and 1.5 GPa. Pressurizing samples from colonies resuscitated from survivors that had been previously exposed to high pressure resulted in substantially greater survivor counts. Experiments were carried out to examine potential interactions between pressure and temperature variables in determining bacterial survival. One generation of survivors previously exposed to 1 GPa was compared with WT samples to investigate survival between 37 and 8°C. The results did not reveal any coupling between acquired high pressure resistance and temperature effects on growth.

Type: Article
Title: Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fmicb.2014.00612
Publisher version: http://dx.doi.org/10.3389/fmicb.2014.00612
Additional information: Copyright © 2014 Hazael, Foglia, Kardzhaliyska, Daniel, Meersman and McMillan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: High pressure biology, Shewanella oneidensis MR-1, bacterial survival, piston cylinder experiments, pressure adaptation studies, variable temperature bacterial growth
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1471078
Downloads since deposit
77Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item