Hazael, R;
Foglia, F;
Kardzhaliyska, L;
Daniel, I;
Meersman, F;
McMillan, P;
(2014)
Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range.
Frontiers in Microbiology
, 5
10.3389/fmicb.2014.00612.
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Abstract
The survival of Shewanella oneidensis MR-1 at up to 1500 MPa was investigated by laboratory studies involving exposure to high pressure followed by evaluation of survivors as the number (N) of colony forming units (CFU) that could be cultured following recovery to ambient conditions. Exposing the wild type (WT) bacteria to 250 MPa resulted in only a minor (0.7 log N units) drop in survival compared with the initial concentration of 108 cells/ml. Raising the pressure to above 500 MPa caused a large reduction in the number of viable cells observed following recovery to ambient pressure. Additional pressure increase caused a further decrease in survivability, with approximately 102 CFU/ml recorded following exposure to 1000 MPa (1 GPa) and 1.5 GPa. Pressurizing samples from colonies resuscitated from survivors that had been previously exposed to high pressure resulted in substantially greater survivor counts. Experiments were carried out to examine potential interactions between pressure and temperature variables in determining bacterial survival. One generation of survivors previously exposed to 1 GPa was compared with WT samples to investigate survival between 37 and 8°C. The results did not reveal any coupling between acquired high pressure resistance and temperature effects on growth.
| Type: | Article |
|---|---|
| Title: | Laboratory investigation of high pressure survival in Shewanella oneidensis MR-1 into the gigapascal pressure range |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3389/fmicb.2014.00612 |
| Publisher version: | http://dx.doi.org/10.3389/fmicb.2014.00612 |
| Additional information: | Copyright © 2014 Hazael, Foglia, Kardzhaliyska, Daniel, Meersman and McMillan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| Keywords: | High pressure biology, Shewanella oneidensis MR-1, bacterial survival, piston cylinder experiments, pressure adaptation studies, variable temperature bacterial growth |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1471078 |
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