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Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces

Aguayo, S; Donos, N; Spratt, D; Bozec, L; (2015) Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces. Journal of Dental Research , 94 (8) pp. 1078-1084. 10.1177/0022034515591485. Green open access

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Abstract

Adhesion of bacteria to dental implant surfaces is the critical initial step in the process of biofilm colonization; however, the specific nanoadhesive interactions occurring during the first contact between bacterial cells and biomaterial substrates remain poorly understood. In this report, we utilize single-cell force spectroscopy to characterize the dynamics of the initial interaction between living Staphylococcus aureus cells and machined titanium surfaces at the nanoscale. Values for maximum adhesion force were found to increase from 0-s (-0.27 ± 0.30 nN) to 60-s (-9.15 ± 0.78 nN) surface delays, with similar results observed for total adhesion work (7.39 ± 2.38 and 988.06 ± 117.08 aJ, respectively). Single unbinding events observed at higher surface delays were modeled according to the wormlike chain model, obtaining molecular contour-length predictions of 314.06 ± 9.27 nm. Average single-bond rupture forces of -0.95 ± 0.04 nN were observed at increased contact times. Short- and long-range force components of bacterial adhesion were obtained by Poisson analysis of single unbinding event peaks, yielding values of -0.75 ± 0.04 and -0.58 ± 0.15 nN, respectively. Addition of 2-mg/mL chlorhexidine to the buffer solution resulted in the inhibition of specific adhesive events but an increased overall adhesion force and work. These results suggest that initial attachment of S. aureus to smooth titanium is mostly mediated by short-range attractive forces observed at higher surface delays.

Type: Article
Title: Nanoadhesion of Staphylococcus aureus onto Titanium Implant Surfaces
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1177/0022034515591485
Publisher version: http://dx.doi.org/10.1177/0022034515591485
Language: English
Additional information: When posting or re-using the article please provide a link to the appropriate DOI for the published version of the article on SAGE Journals (http://online.sagepub.com).
Keywords: atomic force microscopy, bacterial adhesion, biophysics, dental implants, microbiology, nanomechanics
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/1469823
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