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3D culture model of fibroblast-mediated collagen creep to identify abnormal cell behaviour

Kureshi, A; Afoke, A; Wohlert, S; Barker, S; Brown, RA; (2015) 3D culture model of fibroblast-mediated collagen creep to identify abnormal cell behaviour. Biomechanics and Modeling in Mechanobiology , 14 (6) pp. 1255-1263. 10.1007/s10237-015-0672-2. Green open access

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Abstract

Native collagen gels are important biomimetic cell support scaffolds, and a plastic compression process can now be used to rapidly remove fluid to any required collagen density, producing strong 3D tissue-like models. This study aimed to measure the mechanical creep properties of such scaffolds and to quantify any enhanced creep occurring in the presence of cells (cell-mediated creep). The test rig developed applies constant creep tension during culture and measures real-time extension due to cell action. This was used to model extracellular matrix creep, implicated in the transversalis fascia (TF) in inguinal hernia. Experiments showed that at an applied tension equivalent to 15% break strength, cell-mediated creep over 24-h culture periods was identified at creep rates of 0.46 and 0.38%/h for normal TF and human dermal fibroblasts, respectively. However, hernia TF fibroblasts produced negligible cell-mediated creep levels under the same conditions. Raising the cell culture temperature from 4 to 37 ◦C was used to demonstrate live cell dependence of this creep. This represents the first in vitro demonstration of TF cell-mediated collagen creep and to our knowledge the first demonstration of a functional, herniarelated cell abnormality.

Type: Article
Title: 3D culture model of fibroblast-mediated collagen creep to identify abnormal cell behaviour
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s10237-015-0672-2
Publisher version: http://dx.doi.org/10.1007/s10237-015-0672-2
Language: English
Additional information: The final publication is available at Springer via http://dx.doi.org/10.1007/s10237-015-0672-2
Keywords: Compressed collagen · Fibroblasts · Creep · Tissue growth · Hernia.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/1469316
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