Rodriguez-Florez, N;
Oyen, ML;
Shefelbine, SJ;
(2014)
Age-related changes in mouse bone permeability.
Journal of Biomechanics
, 47
(5)
pp. 1110-1116.
10.1016/j.jbiomech.2013.12.020.
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Abstract
The determination of lacunar-canalicular permeability is essential for understanding local fluid flow in bone, which may indicate how bone senses changes in the mechanical environment to regulate mechano-adaptation. The estimates of lacunar-canalicular permeability found in the literature vary by up to eight orders of magnitude, and age-related permeability changes have not been measured in non-osteonal mouse bone. The objective of this study is to use a poroelastic approach based on nanoindentation data to characterize lacunar-canalicular permeability in murine bone as a function of age. Nine wild type C57BL/6 mice of different ages (2, 7 and 12 months) were used. Three tibiae from each age group were embedded in epoxy resin, cut in half and indented in the longitudinal direction in the mid-cortex using two spherical fluid indenter tips (R=238 μm and 500 μm). Results suggest that the lacunar-canalicular intrinsic permeability of mouse bone decreases from 2 to 7 months, with no significant changes from 7 to 12 months. The large indenter tip imposed larger contact sizes and sampled larger ranges of permeabilities, particularly for the old bone. This age-related difference in the distribution was not seen for indents with the smaller radius tip. We conclude that the small tip effectively measured lacunar-canalicular permeability, while larger tip indents were influenced by vascular permeability. Exploring the age-related changes in permeability of bone measured by nanoindentation will lead to a better understanding of the role of fluid flow in mechano-transduction. This understanding may help indicate alterations in bone adaptation and remodeling.
| Type: | Article |
|---|---|
| Title: | Age-related changes in mouse bone permeability |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jbiomech.2013.12.020 |
| Publisher version: | http://dx.doi.org/10.1016/j.jbiomech.2013.12.020 |
| Language: | English |
| Additional information: | Copyright © 2013 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Keywords: | Age, Murine bone, Nanoindentation, Permeability, Poroelasticity, Adaptation, Physiological, Aging, Animals, Bone and Bones, Elasticity, Female, Mice, Mice, Inbred C57BL, Permeability, Tibia |
| UCL classification: | UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1469139 |
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