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Telomeres and centromeres have interchangeable roles in promoting meiotic spindle formation

Fennell, A; Fernández-Álvarez, A; Tomita, K; Cooper, JP; (2015) Telomeres and centromeres have interchangeable roles in promoting meiotic spindle formation. Journal of Cell Biology , 208 (4) pp. 415-428. 10.1083/jcb.201409058. Green open access

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Abstract

Telomeres and centromeres have traditionally been considered to perform distinct roles. During meiotic prophase, in a conserved chromosomal configuration called the bouquet, telomeres gather to the nuclear membrane (NM), often near centrosomes. We found previously that upon disruption of the fission yeast bouquet, centrosomes failed to insert into the NM at meiosis I and nucleate bipolar spindles. Hence, the trans-NM association of telomeres with centrosomes during prophase is crucial for efficient spindle formation. Nonetheless, in approximately half of bouquet-deficient meiocytes, spindles form properly. Here, we show that bouquet-deficient cells can successfully undergo meiosis using centromere-centrosome contact instead of telomere-centrosome contact to generate spindle formation. Accordingly, forced association between centromeres and centrosomes fully rescued the spindle defects incurred by bouquet disruption. Telomeres and centromeres both stimulate focal accumulation of the SUN domain protein Sad1 beneath the centrosome, suggesting a molecular underpinning for their shared spindle-generating ability. Our observations demonstrate an unanticipated level of interchangeability between the two most prominent chromosomal landmarks.

Type: Article
Title: Telomeres and centromeres have interchangeable roles in promoting meiotic spindle formation
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1083/jcb.201409058
Publisher version: http://dx.doi.org/10.1083/jcb.201409058
Language: English
Additional information: This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Keywords: Cell Cycle Proteins, Centromere, Centrosome, Chromatin, Chromosomes, Fungal, Dyneins, Endoribonucleases, Green Fluorescent Proteins, Meiosis, Nuclear Envelope, Nuclear Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Spindle Apparatus, Telomere, Telomere-Binding Proteins
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/1469035
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