Lötvall, J;
Hill, AF;
Hochberg, F;
Buzás, EI;
Di Vizio, D;
Gardiner, C;
Gho, YS;
... Théry, C; + view all
(2014)
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
Journal of Extracellular Vesicles
, 3
(1)
, Article 26913. 10.3402/jev.v3.26913.
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Abstract
Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.
| Type: | Article |
|---|---|
| Title: | Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles |
| Location: | Sweden |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3402/jev.v3.26913 |
| Publisher version: | http://dx.doi.org/10.3402/jev.v3.26913 |
| Additional information: | © 2014 Jan Lotvall et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Ectosomes, exosomes, extracellular RNA, extracellular vesicles, microparticles, microvesicles |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1468494 |
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