UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency.

Tagalakis, AD; Castellaro, S; Zhou, H; Bienemann, A; Munye, MM; McCarthy, D; White, EA; (2015) A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. Int J Nanomedicine , 10 2673 - 2683. 10.2147/IJN.S78935. Green open access

[thumbnail of IJN-78935-a-method-for-concentrating-lipid-peptide-dna-and-sirna-nanoc_040115.pdf] PDF
IJN-78935-a-method-for-concentrating-lipid-peptide-dna-and-sirna-nanoc_040115.pdf

Download (2MB)

Abstract

Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research. Difficulties with formulating the nanoparticles for uniformity and stability at concentrations required for in vivo and clinical use are limiting their progression in these areas. Here, we report a simple but effective method of formulating monodisperse nanocomplexes from a ternary formulation of lipids, targeting peptides, and nucleic acids at a low starting concentration of 0.2 mg/mL of DNA, and we then increase their concentration up to 4.5 mg/mL by reverse dialysis against a concentrated polymer solution at room temperature. The nanocomplexes did not aggregate and they had maintained their biophysical properties, but, importantly, they also mediated DNA transfection and siRNA silencing in cultured cells. Moreover, concentrated anionic nanocomplexes administered by convection-enhanced delivery in the striatum showed efficient silencing of the β-secretase gene BACE1. This method of preparing nanocomplexes could probably be used to concentrate other nonviral formulations and may enable more widespread use of nanoparticles in vivo.

Type: Article
Title: A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency.
Location: New Zealand
Open access status: An open access version is available from UCL Discovery
DOI: 10.2147/IJN.S78935
Publisher version: http://dx.doi.org/10.2147/IJN.S78935
Language: English
Additional information: This work is published by Dove Medical Press Limited, and licensed under a Creative Commons Attribution License. The full terms of the License are available at http://creativecommons.org/licenses/by/4.0/. The license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: DNA, anionic liposome, concentration, nanoparticles, siRNA, targeted gene delivery
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1466965
Downloads since deposit
132Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item