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Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase

Deszcz, D; Affaticati, P; Ladkau, N; Gegel, A; Ward, JM; Hailes, HC; Dalby, PA; (2015) Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase. The FEBS Journal , 282 (13) pp. 2512-2526. 10.1111/febs.13293. Green open access

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Abstract

We have analysed the natural evolution of transaminase structure and sequence between an α-transaminase serine-pyruvate aminotransferase, and an ω-transaminase from Chromobacterium violaceum with <20% sequence identity, and identified the active-site regions which are least conserved structurally. We also show that these structural changes correlate strongly with transaminase substrate specificity during evolution and therefore might normally be presumed to be essential determinants of substrate specificity. However, key residues are often conserved spatially during evolution and yet come from within a different region of the sequence via structural reorganisations. Here we also show that α-transaminase-type serine-pyruvate aminotransferase activity, can be engineered into the CV2025 ω-transaminase scaffold with any one of many possible single point mutations at three key positions, without the requirement for significant backbone remodeling, or repositioning of the residue from a different region of sequence. This finding has significant implications for enzyme redesign in which solutions to substrate specificity changes may be found that are significantly more efficient than by engineering in all sequence and structure determinants identified by correlation to substrate specificity. This article is protected by copyright. All rights reserved.

Type: Article
Title: Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/febs.13293
Publisher version: http://dx.doi.org/10.1111/febs.13293
Language: English
Additional information: This is the peer reviewed version of the following article: Deszcz, D; Affaticati, P; Ladkau, N; Gegel, A; Ward, JM; Hailes, HC; Dalby, PA; (2015) Single active-site mutants are sufficient to enhance serine:pyruvate α-transaminase activity in an ω-transaminase. The FEBS Journal, 282 (13) pp. 2512-2526, which has been published in final form at: http://dx.doi.org/10.1111/febs.13293. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html#terms).
Keywords: Aminotransferase, biocatalysis, directed evolution, substrate specificity, transaminase.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1466729
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