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Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery

Battigelli, A; Russier, J; Bianco, A; Da Ros, T; Prato, M; Kostarelos, K; Wang, JT-W; (2013) Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery. Small , 9 (21) 3610 - 3619. 10.1002/smll.201300264. Green open access

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Abstract

A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material. Carbon nanotubes functionalized by amidation or click chemistry with dendrons of different generations possessing ammonium or guanidinium groups are synthesized and complexed to siRNA. The assessment of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence genes shows them to be promising carriers for genetic material.

Type: Article
Title: Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/smll.201300264
Publisher version: http://dx.doi.org/10.1002/smll.201300264
Additional information: © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. The copyright line for this article was changed on 16 Sept 2014 after original online publication.
Keywords: Carbon nanotubes; Cationic dendrons; siRNA, RNA delivery;Gene therapy
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/1466163
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