Pesce, G; Volpe, G; Marago, OM; Jones, PH; Gigan, S; Sasso, A; Volpe, G; (2015) A Step-by-step Guide to the Realisation of Advanced Optical Tweezers. Journal of the Optical Society of America B , 32 (5) B84- B98. 10.1364/JOSAB.32.000B84.

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Abstract

Since the pioneering work of Arthur Ashkin, optical tweezers have become an indispensable tool for contactless manipulation of micro- and nanoparticles. Nowadays optical tweezers are employed in a myriad of applications demonstrating their importance. While the basic principle of optical tweezers is the use of a strongly focused laser beam to trap and manipulate particles, ever more complex experimental set-ups are required in order to perform novel and challenging experiments. With this article, we provide a detailed step-by-step guide for the construction of advanced optical manipulation systems. First, we explain how to build a single-beam optical tweezers on a home-made microscope and how to calibrate it. Improving on this design, we realize a holographic optical tweezers, which can manipulate independently multiple particles and generate more sophisticated wavefronts such as Laguerre-Gaussian beams. Finally, we explain how to implement a speckle optical tweezers, which permits one to employ random speckle light fields for deterministic optical manipulation. OCIS codes: (350.4855) Optical tweezers or optical manipulation; (120.4640) Optical instruments; (090.1760) Computer holography; (030.6140) Speckle; (170.4520) Optical confinement and manipulation;

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