Nans, A;
Saibil, HR;
Hayward, RD;
(2014)
Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography.
Cellular Microbiology
, 16
(10)
pp. 1457-1472.
10.1111/cmi.12310.
Preview |
Text
Nans_Pathogen-host reorganization_ during_ Chlamydia_ invasion.pdf Download (2MB) | Preview |
Abstract
Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualized in three-dimensional detail. We have captured snapshots of the early stages of bacterial-mediated endocytosis in situ by exploiting the small size of chlamydial elementary bodies (EBs) for whole-cell cryo-electron tomography. Chlamydiae are obligate intracellular bacteria that infect eukaryotic cells and cause sexually transmitted infections and trachoma, the leading cause of preventable blindness. We demonstrate that Chlamydia trachomatis LGV2 EBs are intrinsically polarized. One pole is characterized by a tubular inner membrane invagination, while the other exhibits asymmetric periplasmic expansion to accommodate an array of type III secretion systems (T3SSs). Strikingly, EBs orient with their T3SS-containing pole facing target cells, enabling the T3SSs to directly contact the cellular plasma membrane. This contact induces enveloping macropinosomes, actin-rich filopodia and phagocytic cups to zipper tightly around the internalizing bacteria. Once encapsulated into tight early vacuoles, EB polarity and the T3SSs are lost. Our findings reveal previously undescribed structural transitions in both pathogen and host during the initial steps of chlamydial invasion.
| Type: | Article |
|---|---|
| Title: | Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/cmi.12310 |
| Publisher version: | http://dx.doi.org/10.1111/cmi.12310 |
| Language: | English |
| Additional information: | Copyright © 2014 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Bacterial Secretion Systems, Cell Line, Tumor, Cell Membrane, Chlamydia Infections, Chlamydia trachomatis, Electron Microscope Tomography, Endocytosis, HeLa Cells, Host-Pathogen Interactions, Humans, Vacuoles |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1460993 |
Archive Staff Only
 |
View Item |
