UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Hepatic arterial spin labelling MRI: an initial evaluation in mice

Ramasawmy, R; Campbell-Washburn, AE; Wells, JA; Johnson, SP; Pedley, RB; Walker-Samuel, S; Lythgoe, MF; (2015) Hepatic arterial spin labelling MRI: an initial evaluation in mice. NMR in Biomedicine , 28 (2) pp. 272-280. 10.1002/nbm.3251. Green open access

[thumbnail of Ramasawmy_et_al-2015-NMR_in_Biomedicine.pdf]
Preview
Text
Ramasawmy_et_al-2015-NMR_in_Biomedicine.pdf

Download (2MB) | Preview

Abstract

The development of strategies to combat hepatic disease and augment tissue regeneration has created a need for methods to assess regional liver function. Liver perfusion imaging has the potential to fulfil this need, across a range of hepatic diseases, alongside the assessment of therapeutic response. In this study, the feasibility of hepatic arterial spin labelling (HASL) was assessed for the first time in mice at 9.4 T, its variability and repeatability were evaluated, and it was applied to a model of colorectal liver metastasis. Data were acquired using flow-sensitive alternating inversion recovery-arterial spin labelling (FAIR-ASL) with a Look-Locker readout, and analysed using retrospective respiratory gating and a T1 -based quantification. This study shows that preclinical HASL is feasible and exhibits good repeatability and reproducibility. Mean estimated liver perfusion was 2.2 ± 0.8 mL/g/min (mean ± standard error, n = 10), which agrees well with previous measurements using invasive approaches. Estimates of the variation gave a within-session coefficient of variation (CVWS) of 7%, a between-session coefficient of variation (CVBS) of 9% and a between-animal coefficient of variation (CVA) of 15%. The within-session Bland-Altman repeatability coefficient (RCWS) was 18% and the between-session repeatability coefficient (RCBS) was 29%. Finally, the HASL method was applied to a mouse model of liver metastasis, in which significantly lower mean perfusion (1.1 ± 0.5 mL/g/min, n = 6) was measured within the tumours, as seen by fluorescence histology. These data indicate that precise and accurate liver perfusion estimates can be achieved using ASL techniques, and provide a platform for future studies investigating hepatic perfusion in mouse models of disease.

Type: Article
Title: Hepatic arterial spin labelling MRI: an initial evaluation in mice
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/nbm.3251
Publisher version: http://dx.doi.org/10.1002/nbm.3251
Language: English
Additional information: © 2014 The Authors. NMR in Biomedicine published by John Wiley & Sons, Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
Keywords: ASL, liver, metastasis, mouse, perfusion, preclinical, repeatability, variability, Animals, Female, Hepatic Artery, Liver Neoplasms, Magnetic Resonance Imaging, Mice, Inbred BALB C, Perfusion, Reproducibility of Results, Spin Labels
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Department of Imaging
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Experimental and Translational Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1459991
Downloads since deposit
120Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item