UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Dual labeling of neural crest cells and blood vessels within chicken embryos using chickGFP neural tube grafting and carbocyanine dye DiI injection

Delalande, JM; Thapar, N; Burns, AJ; (2015) Dual labeling of neural crest cells and blood vessels within chicken embryos using chickGFP neural tube grafting and carbocyanine dye DiI injection. Journal of Visualized Experiments , 99 , Article e52514. 10.3791/52514. Green open access

[img]
Preview
Text
burns_jove-protocol-52514-dual-labeling-neural-crest-cells-blood-vessels-within-chicken-embryos.pdf

Download (1MB) | Preview

Abstract

All developing organs need to be connected to both the nervous system (for sensory and motor control) as well as the vascular system (for gas exchange, fluid and nutrient supply). Consequently both the nervous and vascular systems develop alongside each other and share striking similarities in their branching architecture. Here we report embryonic manipulations that allow us to study the simultaneous development of neural crest-derived nervous tissue (in this case the enteric nervous system), and the vascular system. This is achieved by generating chicken chimeras via transplantation of discrete segments of the neural tube, and associated neural crest, combined with vascular DiI injection in the same embryo. Our method uses transgenic chickGFP embryos for intraspecies grafting, making the transplant technique more powerful than the classical quail-chick interspecies grafting protocol used with great effect since the 1970s. ChickGFP-chick intraspecies grafting facilitates imaging of transplanted cells and their projections in intact tissues, and eliminates any potential bias in cell development linked to species differences. This method takes full advantage of the ease of access of the avian embryo (compared with other vertebrate embryos) to study the co-development of the enteric nervous system and the vascular system.

Type: Article
Title: Dual labeling of neural crest cells and blood vessels within chicken embryos using chickGFP neural tube grafting and carbocyanine dye DiI injection
Location: USA
Open access status: An open access version is available from UCL Discovery
DOI: 10.3791/52514
Publisher version: http://dx.doi.org/10.3791/52514
Language: English
Additional information: Copyright © 2015 Creative Commons Attribution 3.0 License
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept
URI: https://discovery.ucl.ac.uk/id/eprint/1449540
Downloads since deposit
103Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item