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Live cell imaging of membrane / cytoskeleton interactions and membrane topology

Chierico, L; Joseph, AS; Lewis, AL; Battaglia, G; (2014) Live cell imaging of membrane / cytoskeleton interactions and membrane topology. Scientific Reports , 4 , Article 6056. 10.1038/srep06056. Green open access

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Abstract

We elucidate the interaction between actin and specific membrane components, using real time live cell imaging, by delivering probes that enable access to components, that cannot be accessed genetically. We initially investigated the close interplay between Phosphatidylinositol 4,5-bisphosphate (PIP2) and the F-actin network. We show that, during the early stage of cell adhesion, PIP2 forms domains within the filopodia membrane. We studied these domains alongside cell spreading and observed that these very closely follow the actin tread-milling. We show that this mechanism is associated with an active transport of PIP2 rich organelles from the cell perinuclear area to the edge, along actin fibers. Finally, mapping other phospholipids and membrane components we observed that the PIP2 domains formation is correlated with sphingosine and cholesterol rafts.

Type: Article
Title: Live cell imaging of membrane / cytoskeleton interactions and membrane topology
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/srep06056
Publisher version: http://dx.doi.org/10.1038/srep06056
Language: English
Additional information: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
Keywords: 3T3 Cells, Actin Cytoskeleton, Actin-Related Protein 2-3 Complex, Actins, Animals, Biological Transport, Active, Cell Adhesion, Cell Line, Cell Membrane, Cell Movement, Cholesterol, Image Processing, Computer-Assisted, Mice, Microscopy, Confocal, Microscopy, Fluorescence, Phosphatidylinositol 4,5-Diphosphate, Protein Structure, Tertiary, Protein Transport, Sphingosine, Surface Properties
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1448822
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