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Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples.

Huy, TQ; Van Chung, P; Thuy, NT; Blanco-Andujar, C; Thanh, NT; (2014) Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples. Faraday Discuss , 175 pp. 73-82. 10.1039/c4fd00152d. Green open access

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Abstract

Pathogen separation is of great significance for precise detection and prevention of disease outbreaks. For the first time, protein A conjugated with chitosan-coated iron oxide nanoparticles was prepared for pathogen separation at low concentrations from liquid samples. Vibrio cholerae O1 (VO1) bacteria were used for testing the effectiveness of this conjugate. Transmission electron microscopy (TEM) was used to confirm the presence of captured VO1. The results showed that, after binding with a specific antibody, the conjugate allows separation of VO1 bacteria from water samples at a concentration as low as 10 cfu mL(-1). Moreover, the conjugate can be used in parallel with conventional or modern diagnostic tests for quick and accurate detection of pathogens.

Type: Article
Title: Protein A-conjugated iron oxide nanoparticles for separation of Vibrio cholerae from water samples.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1039/c4fd00152d
Publisher version: http://dx.doi.org/10.1039/c4fd00152d
Language: English
Additional information: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence
Keywords: Bacteriological Techniques, Ferric Compounds, Nanoparticles, Particle Size, Staphylococcal Protein A, Surface Properties, Vibrio cholerae, Water Microbiology
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy
URI: https://discovery.ucl.ac.uk/id/eprint/1448056
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