Said, J;
Walker, M;
Parsons, D;
Stapleton, P;
Beezer, AE;
Gaisford, S;
(2014)
An in vitro test of the efficacy of an anti-biofilm wound dressing.
International Journal of Pharmaceutics
, 474
(1-2)
pp. 177-181.
10.1016/j.ijpharm.2014.08.034.
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Abstract
Broad-spectrum antimicrobial agents, such as silver, are increasingly being formulated into medicated wound dressings in order to control colonization of wounds by opportunistic pathogens. Medicated wound dressings have been shown in-vitro to be effective against planktonic cultures, but in-vivo bacteria are likely to be present in biofilms, which makes their control and eradication more challenging. Recently, a functional wound dressing (AQUACEL(®) Ag+ Extra™ (AAg + E)) has been developed that in addition to silver contains two agents (ethylenediaminetetraacetic acid (EDTA) and benzethonium chloride (BC)) designed to disrupt biofilms. Here, the efficacy of AAg + E is demonstrated using a biofilm model developed in an isothermal microcalorimeter. The biofilm was seen to remain viable in the presence of unmedicated dressing, silver-containing dressing or silver nitrate solution. In the presence of AAg + E, however, the biofilm was eradicated. Control experiments showed that neither EDTA nor BC alone had a bactericidal effect, which means it is the synergistic action of EDTA and BC disrupting the biofilm with silver being bactericidal that leads to the product's efficacy.
| Type: | Article |
|---|---|
| Title: | An in vitro test of the efficacy of an anti-biofilm wound dressing |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.ijpharm.2014.08.034 |
| Publisher version: | http://dx.doi.org/10.1016/j.ijpharm.2014.08.034 |
| Language: | English |
| Additional information: | © 2014. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. |
| Keywords: | Biofilm, Isothermal calorimetry, Staphylococcus aureus, Wound dressings, silver, Anti-Bacterial Agents, Benzethonium, Biofilms, Carboxymethylcellulose Sodium, Edetic Acid, Microbial Sensitivity Tests, Occlusive Dressings, Silver, Staphylococcus aureus, Wound Infection, Wounds and Injuries |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1447433 |
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