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Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots

Yaghini, E; Pirker, KF; Kay, CW; Seifalian, AM; MacRobert, AJ; (2014) Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots. Small , 10 (24) pp. 5106-5115. 10.1002/smll.201401209. Green open access

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Abstract

Photocatalytic generation of reactive oxygen species (ROS) from quantum dots (QDs) has been widely reported yet quantitative studies of ROS formation and their quantum yields are lacking. This study investigates the generation of ROS by water soluble PEGylated CdSe/ZnS QDs with red emission. PEGylation of QDs is commonly used to confer water solubility and minimise uptake by organs of the reticuloendothelial system; therefore studies of ROS formation are of biomedical relevance. Using non-photolytic visible wavelength excitation, the superoxide anion radical is shown to be the primary ROS species generated with a quantum efficiency of 0.35%. The yield can be significantly enhanced in the presence of the electron donor, nicotinamide adenine dinucleotide (NADH), as demonstrated by oxygen consumption measurements and electron paramagnetic resonance spectroscopy with in situ illumination. Direct production of singlet oxygen is not detectable from the QDs alone. A comparison is made with ROS generation by the same QDs complexed with a sulfonated phthalocyanine which can generate singlet oxygen via Förster resonance energy transfer between the QDs and the phthalocyanine.

Type: Article
Title: Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/smll.201401209
Publisher version: http://dx.doi.org/10.1002/smll.201401209
Language: English
Additional information: This is the peer reviewed version of the following article: Yaghini, E; Pirker, KF; Kay, CW; Seifalian, AM; MacRobert, AJ; (2014) Quantification of reactive oxygen species generation by photoexcitation of PEGylated quantum dots. Small , 10 (24) pp. 5106-5115, which has been published in final form at http://dx.doi.org/10.1002/smll.201401209. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-820227.html#terms).
Keywords: EPR spectroscopy, quantum dots, singlet oxygen, spin trapping, superoxide anion radicals, Electron Spin Resonance Spectroscopy, Light, Oxygen Consumption, Polyethylene Glycols, Quantum Dots, Reactive Oxygen Species
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1447279
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