Hudson, N;
Powner, MB;
Sarker, MH;
Burgoyne, T;
Campbell, M;
Ockrim, ZK;
Martinelli, R;
... Turowski, P; + view all
(2014)
Differential apicobasal VEGF signaling at vascular blood-neural barriers.
Dev Cell
, 30
(5)
541 - 552.
10.1016/j.devcel.2014.06.027.
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Abstract
The vascular endothelium operates in a highly polarized environment, but to date there has been little exploration of apicobasal polarization of its signaling. We show that VEGF-A, histamine, IGFBP3, and LPA trigger unequal endothelial responses when acting from the circulation or the parenchymal side at blood-neural barriers. For VEGF-A, highly polarized receptor distribution contributed to distinct signaling patterns: VEGFR2, which was found to be predominantly abluminal, mediated increased permeability via p38; in contrast, luminal VEGFR1 led to Akt activation and facilitated cytoprotection. Importantly, such differential apicobasal signaling and VEGFR distribution were found in the microvasculature of brain and retina but not lung, indicating that endothelial cells at blood-neural barriers possess specialized signaling compartments that assign different functions depending on whether an agonist is tissue or blood borne.
| Type: | Article |
|---|---|
| Title: | Differential apicobasal VEGF signaling at vascular blood-neural barriers. |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.devcel.2014.06.027 |
| Publisher version: | http://dx.doi.org/10.1016/j.devcel.2014.06.027 |
| Language: | English |
| Additional information: | © 2014 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). |
| Keywords: | Animals, Blood-Brain Barrier, Endothelium, Vascular, Male, Mice, Mice, Inbred C57BL, Microcirculation, Neurons, Permeability, Rats, Rats, Inbred Lew, Rats, Wistar, Signal Transduction, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor Receptor-2, p38 Mitogen-Activated Protein Kinases |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1447041 |
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