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Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium.

Wavre-Shapton, ST; Meschede, IP; Seabra, MC; Futter, CE; (2014) Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium. J Cell Sci , 127 pp. 3852-3861. 10.1242/jcs.154757. Green open access

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Abstract

Defects in phagocytosis and degradation of photoreceptor outer segments (POS) by the retinal pigment epithelium (RPE) are associated with aging and retinal disease. The daily burst of rod outer segment (ROS) phagocytosis by the RPE provides a unique opportunity to analyse phagosome processing in vivo. In mouse retinae phagosomes containing stacked rhodopsin-rich discs were identified by immuno-electron microscopy. Early apical phagosomes stained with both cytoplasmic and intradiscal domain rhodopsin antibodies. During phagosome maturation a remarkably synchronised loss of the cytoplasmic epitope coincided with movement to the cell body and preceded phagosome:lysosome fusion and disc degradation. Loss of the intradiscal rhodopsin epitope and disc digestion occurred upon fusion with cathepsin D-positive lysosomes. The same sequential stages of phagosome maturation were identified in cultured RPE and macrophages challenged with isolated POS. Loss of the cytoplasmic rhodopsin epitope was insensitive to pH but sensitive to protease inhibition and co-incided with interaction of phagosomes with endosomes. Thus, during prelysosomal maturation of ROS-containing phagosomes limited rhodopsin processing occurs upon interaction with endosomes. This potentially provides a sensitive readout of phagosome:endosome interactions applicable to multiple phagocytes.

Type: Article
Title: Phagosome maturation during endosome interaction revealed by partial rhodopsin processing in retinal pigment epithelium.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.154757
Publisher version: http://dx.doi.org/10.1242/​jcs.154757
Language: English
Additional information: © 2014. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/1447040
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